Location: Baltimore, Maryland
Internal Number: 108897-en_US
The Pathology Department is seeking a proficient Research Technologist with a firm understanding of techniques and a solid foundation in the underlying science.
Under minimal supervision, performs research experiments on mouse and human research projects in a Bio Safety Level 2 setting. Using established procedures and protocols, performs experiments which may include: mouse handling techniques including in vivo injections, skin grafting, peripheral blood collection, and tissue harvesting; animal husbandry including phenotyping/genotyping transgenic mouse strains; multi-parameter flow cytometry; cell culture; DNA and RNA extractions; and data analysis. Flow cytometry and tissue culture of human peripheral blood cells may also be performed. The individual will participate in the planning of experiments and interpreting of results. Responsible for routine operational activities such as: maintaining up to date records of experiments, reviewing inventory of laboratory supplies, and ordering laboratory equipment and supplies. Identifies and resolves problems and uses analytical and decision-making skills to implement assigned projects.
Specific Duties & Responsibilities
- Performs miscellaneous related duties as assigned.
- Performs general lab housekeeping and maintaining safety records.
- Conducts preventive maintenance of equipment to include maintaining and troubleshooting equipment, making routine repairs or notifying service personnel when more complex problems occur.
- Responsible for routine operational activities such as ordering supplies and materials, reviewing inventory, maintaining up to date records of tests, etc.
- Maintains accurate and detailed records of experimental procedures and results. Exchanges routine information in an appropriate manner requiring good oral and written communication. Regular interaction with students, vendors, post-doctoral fellows, faculty members, and other clinical fellows.
- Manages mouse colony, including transgenic mouse strain breeding, ordering of mice from commercial vendors, and attending to time-sensitive issues that arise with Research Animal Resources staff. Monitors breeding and weaning of new litters, keeps accurate electronic records. Genotypes new litters at appropriate age using PCR and/or flow cytometry.
- Performs clinical and/or research lab tests and experiments. Follows established procedures and protocols, utilize appropriate techniques to perform laboratory tests and/or experiments which may include development of immunological assay and handling of animal, cell culture, as well as biological technology.
Special Knowledge, Skills, & Abilities
- Knowledge of good lab practices. Good computational skills. Good oral and written communication skills.
- While performing the duties of this job, the employee may remain in a stationary position (sitting or standing) up to 20% of the time. Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus. The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides. Constantly repositions self to perform duties at laboratory work stations, gathering specimens, and loading equipment.
- Working with animals (mice), including handling techniques, injections, euthanasia, bleeding, and minor procedures in the laboratory as well as facilities of the Research Animal Resources in multiple locations on campus.
- Most activities may be scheduled during normal business hours (Monday-Friday, 8 AM – 5 PM). However, strict scheduling may not be possible on a daily basis, and may depend on the completion of time-sensitive aspects of experiments and scheduling time on shared equipment (e.g. flow cytometry core equipment). Occasional weekend tasks may be required.
- Prolonged vision requirements including viewing computer screen, paper reports/documents, charts and results. Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).
- Laboratory environment - Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed. Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas. Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area. Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment. Research areas may include animal, human, human products, DNA, and radiation protocols. Use of personal protective equipment may be advised or required. May transport equipment and supplies usually less than 40 pounds from one area to another. In some areas work space is limited.
- Staff may be required to complete annual competency review, and must ensure compliance with Johns Hopkins Policy, Laboratory Policy & Procedure, Health, Safety & Environment regulations, and all applicable privacy & confidentiality laws/practices.
- Bachelor's Degree in Biology, Chemistry or related field.
- Some related post-undergraduate work experience preferred.
- Minimum of 1 year related clinical and/or research laboratory experience performing relevant or related techniques.
- Experience with mouse handling and/or husbandry.
- Experience with multi-parameter flow cytometry.
Classified Title: Research Technologist
Starting Salary Range: $15.00-$20.40-$25.50 HRLY (Commensurate with experience)
Employee group: Full Time
Schedule: M-F, 8:30am-5:00pm (start/end times flexible)
Exempt Status: Non-Exempt
Location: School of Medicine Campus
Department name: SOM Pat Immunology
Personnel area: School of Medicine
The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.
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All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at email@example.com. For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit accessibility.jhu.edu.
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The following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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