Location: Baltimore, Maryland
Internal Number: 108299-en_US
We are seeking a Research Technologist to support daily operations of a research laboratory that studies interactions between transcription factors and chromatin and how that interaction changes chromatin structure, dynamics, and accessibility by other molecules. We use high-resolution methods (NMR, X-ray crystallography) complemented with biophysical and biochemical experiments to probe the interaction between these proteins and nucleosomes in vitro. Following adequate training, you will be responsible for the preparation and purification of protein and nucleic acid samples and aid in the development, execution, and troubleshooting of various established and novel in vitro assays for biomolecular interactions. You will also help in ordering and maintenance of laboratory equipment, supplies, reagents, and other materials. If desired, you will have the opportunity to actively participate in the design of research goals and experiments, to assist with the preparation of grants and manuscripts for publication as well as to present your research progress at conferences.
Specific Duties & Responsibilities
- Preparation, purification, and analysis of protein and DNA samples.
- Execution and troubleshooting of various biochemical and biophysical assays to probe biomolecular interactions.
- Operation of basic and advanced laboratory equipment (liquid chromatography, thermocycler, incubator, centrifuge, UV/Vis spectrometer, fluorimeter, autoclave, and other basic equipment).
- Help with general maintenance of laboratory equipment, ordering of materials and supplies, preparation of reagents and solutions following standard procedures.
- Research project design and manuscript preparation.
Special Knowledge, Skills & Abilities
- Knowledge of standard molecular biology techniques.
- Knowledge of basic laboratory methods, procedures, and techniques in molecular biology and biochemistry.
- Knowledge of Microsoft Office and experience with Adobe Creative Suite.
- Bachelor's Degree in Biology, Chemistry or related field.
- Some related post-undergraduate training experience is preferred.
- Experience with protein expression, purification, and mutagenesis and nucleic acid preparation.
- Excellent time management, organizational, and communication skills.
- Experience with nucleosome preparation, fluorescence-based binding assays, NMR spectroscopy or other biophysical techniques is a plus.
Classified Title: Research Technologist
Starting Salary Range: $15.00-$20.40-$25.50 HRLY (Commensurate with experience)
Employee group: Full Time
Schedule: M-F 8:30am-5:00pm
Exempt Status: Non-Exempt
Location: Homewood Campus
Department name: Biophysics
Personnel area: School of Arts & Sciences
The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.
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